The Translational Proteomics Center at Boston Children’s Hospital, directed by Professor Hanno Steen, delivers high-throughput, automated proteomics analysis for human body fluids, including plasma, serum, urine, saliva, and tracheal aspirates from patients ranging from neonates to adults. Using state-of-the-art Bruker timsTOF mass spectrometers and Opentrons liquid handling robots, the Center performs deep proteomic profiling while requiring only small microliter sample volumes, preserving precious clinical material. By combining advanced instrumentation, reproducible workflows, and expert training, it enables large-scale cohort studies, pilot projects, and collaborative research, accelerating translational discoveries and standardizing data generation across diverse clinical and research applications.